Biological Safety Cabinets, enclosed containers and other engineering controls are designed to remove hazardous biological materials. The biological safety cabinet is the principal device used to provide containment of infectious splashes or aerosols generated by many microbiological procedures. The equipment is widely applicable and is used in biomedicine, microbiology and gene recombination. Our Biological Safety Cabinets have the following technical specification:
Class II Biosafety Cabinet Type A1 & A2 are designed for the security and protection of operator’s product and environment from pathogenic agents and microorganisms transmitted in respiratory way and also for creation of a bacterial dust free air environment. It has a common plenum from which 30% of air is exhausted, and 70% re-circulated to the work area as part of the downflow.
Type A cabinets drain out air directly back to the laboratory, and they may contain positive pressure contaminated plenums. When toxic chemicals must be employed as an adjunct to microbiological processes, these cabinets should not be used. Exhaust HEPA filtration only.
The Class II Type A1 consists of the positively-pressurized contaminated plenum bordering the ambient environment, and therefore is less safer than the Class II Type A2 that has a negative pressure surrounding the positively pressurized contaminated plenum.
In any unlikely event of a leakage on the positive plenum, the leaking aerosol will be pulled by the negative pressure back to the positive plenum, which prevents it from leaking out. On the grounds of safety, the Type A1 design is now considered obsolete. In the A2 cabinet, approximately 70% of air from the positive plenum is re-circulated as downflow.
Laboratory Biosafety Manual.
Second Edition (revised). Interim guidelines. World Health Organization. p32.
Class | Inflow Velocity m/s |
Recirculation of Air (%) | Exhaust Air (%) |
Control Plenum surrounded By | Exhaust Alternatives | Biosafety Level |
Class I | US: 038 EN: 0.70 |
0 | 100 | Outside Air | Inside room / |
1, 2 & 3 |
Class II A1 |
US: 038 EN: 0.40 |
70 | 30 | Outside Air | Inside room / Hard duct |
1, 2 & 3 |
Class II A2 |
US: 050 EN: 0.40 |
70 | 30 | Negative Plenum | Inside room / Hard duct |
1, 2 & 3 |
Class II B1 |
US: 050 EN: 0.40 |
30 | 70 | Negative Plenum | Hard Duct Only | 1, 2 & 3 |
Class II B2 |
US: 050 EN:NA |
0 | 100 | Negative Plenum | Hard Duct Only | 1, 2 & 3 |
Class III | Closed:* >0.5”WC |
0 | 100 | Negative Plenum | Inside Room/Hard Duct | 1, 2, 3 & 4 |
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